illumina NXTR XT樣本制備試劑盒 *

illumina NXTR XT樣本制備試劑盒 *

illumina 15032350

illumina 15032350 NXTR XT樣本制備試劑盒

NXTR XT樣本制備試劑盒（24樣本 第一盒）

Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354

NXTR XT標(biāo)簽試劑盒V2（套件A 96標(biāo)簽 192樣本）15052163

Next-Generation sequencing

Total RNA was extracted from each patient’s plasma sample, and cDNA synthesis followed. The QIAamp Viral RNA Mini Kit (Cat. #52904, Qiagen, Gaithersburg, MD) and the SuperScript?6?4 IV First-Strand Synthesis System (Cat. # 18091050, Invitrogen, Carlsbad, CA) were used respectively and according to manufacturers’ instructions. Multiple sets of HIV-1 specific primer pairs were used to target and amplify using polymerase-chain-reaction (PCR) the protease (PR), reverse transcriptase (RT), integrase (int), and envelope (env) HIV-1 genes (~43% of genome)36. Library preparation was completed with Nextera XT Library Prep (Cat. # 15032350, Illumina, Dan Diego, CA). Samples were then sequenced on eight runs on an Illumina MiSeq platform using the MiSeq v2 (300 cycles) chemistry (Cat. # MS-102–2002, Illumina). Both library prep and sequencing were completed according to the manufacturer’s instructions. All DNA sequence files are available from the GenBank database under SRA accession: PRJNA517147.